2024

Lukas Schartel, Cosimo Jann, Anna Wierczeiko, Tamer Butto, Stefan Mündnich, Virginie Marchand, Yuri Motorin, Mark Helm, Susanne Gerber, Edward A. Lemke (2024) Selective RNA pseudouridinylation in situ by circular gRNAs in designer organelles, Nature Communications 2024 15:1 15(1), p. 1-10, Nature Publishing Group, url, doi:10.1038/s41467-024-53403-1

Mikhail E. Sushkin, Marius Jung, Edward A. Lemke (2024) Tuning the Functionality of Designer Translating Organelles with Orthogonal tRNA Synthetase/tRNA Pairs, Journal of molecular biology, J Mol Biol, url, doi:10.1016/J.JMB.2024.168728

Cosimo Jann, Sabrina Giofré, Rajanya Bhattacharjee, Edward A. Lemke (2024) Cracking the Code: Reprogramming the Genetic Script in Prokaryotes and Eukaryotes to Harness the Power of Noncanonical Amino Acids, Chemical Reviews, American Chemical Society, url, doi:doi.org/10.1021/acs.chemrev.3c00878

Mikhail E. Sushkin, Marius Jung, Edward A. Lemke (2024) Tuning the functionality of designer translating organelles with orthogonal tRNA synthetase/tRNA pairs, Journal of Molecular Biology, p. 168728, Academic Press, url, doi:10.1016/J.JMB.2024.168728

Yuanyun Ao, Jonathan R. Grover, Levi Gifford, Yang Han, Guohua Zhong, Revansiddha Katte, Wenwei Li, Rajanya Bhattacharjee, Baoshan Zhang, Stephanie Sauve, Wenyi Qin, Dibya Ghimire, Md Anzarul Haque, James Arthos, Mahmoud Moradi, Walther Mothes, Edward A. Lemke, Peter D. Kwong, Gregory B. Melikyan, Maolin Lu (2024) Bioorthogonal click labeling of an amber-free HIV-1 provirus for in-virus single molecule imaging, Cell Chemical Biology 31(3), p. 487-501.e7, Cell Press, doi:10.1016/J.CHEMBIOL.2023.12.017

Dorothee Dormann, Edward Anton Lemke (2024) Adding intrinsically disordered proteins to biological ageing clocks, Nature Cell Biology 2024 26:6 26(6), p. 851-858, Nature Publishing Group, url, doi:10.1038/s41556-024-01423-w

Edward A. Lemke, M. Madan Babu, Richard W. Kriwacki, Tanja Mittag, Rohit V. Pappu, Peter E. Wright, Julie D. Forman-Kay (2024) Intrinsic disorder: A term to define the specific physicochemical characteristic of protein conformational heterogeneity, Molecular Cell 84(7), p. 1188-1190, Cell Press, doi:10.1016/J.MOLCEL.2024.02.024

2023

Mikhail E. Sushkin, Christine Koehler, Edward A. Lemke (2023) Remodeling the cellular stress response for enhanced genetic code expansion in mammalian cells, Nature Communications 2023 14:1 14(1), p. 1-14, Nature Publishing Group, url, doi:10.1038/s41467-023-42689-2

Tom Scheidt, Hao Ruan, Miao Yu, Edward A. Lemke (2023) Stressing the role of a short linear motif in ataxin-2 condensation, Molecular Cell 83(12), p. 1961-1963, Cell Press, doi:10.1016/J.MOLCEL.2023.05.024

Christopher D. Reinkemeier, Edward A. Lemke (2023) Synthetic Organelles for Multiple mRNA Selective Genetic Code Expansions in Eukaryotes, Methods in Molecular Biology 2563, p. 341-369, Humana Press Inc., url, doi:10.1007/978-1-0716-2663-4_17/FIGURES/7

Sören Kirchgäßner, Michael B. Braun, Natascha Bartlick, Cengiz Koç, Christopher D. Reinkemeier, Edward A. Lemke, Thilo Stehle, Dirk Schwarzer (2023) Synthesis, Biochemical Characterization, and Genetic Encoding of a 1,2,4-Triazole Amino Acid as an Acetyllysine Mimic for Bromodomains of the BET Family, Angewandte Chemie International Edition 62(12), p. e202215460, John Wiley & Sons, Ltd, url, doi:10.1002/ANIE.202215460

Christine Koehler, Paul F. Sauter, Benedikt Klasen, Christopher Waldmann, Stefanie Pektor, Nicole Bausbacher, Edward A. Lemke, Matthias Miederer (2023) Genetic Code Expansion for Site-Specific Labeling of Antibodies with Radioisotopes, ACS Chemical Biology 18(3), p. 443-448, American Chemical Society, url, doi:10.1021/ACSCHEMBIO.2C00634/ASSET/IMAGES/LARGE/CB2C00634_0003.JPEG

Yuanyun Ao, Jonathan R. Grover, Yang Han, Guohua Zhong, Wenyi Qin, Dibya Ghimire, Anzarul Haque, Rajanya Bhattacharjee, Baoshan Zhang, James Arthos, Edward A. Lemke, Peter D. Kwong, Maolin Lu (2023) An intact amber-free HIV-1 system for in-virus protein bioorthogonal click labeling that delineates envelope conformational dynamics, bioRxiv, p. 2023.02.28.530526, Cold Spring Harbor Laboratory, url, doi:10.1101/2023.02.28.530526

Ganesh Agam, Christian Gebhardt, Milana Popara, Rebecca Mächtel, Julian Folz, Benjamin Ambrose, Neharika Chamachi, Sang Yoon Chung, Timothy D. Craggs, Marijn de Boer, Dina Grohmann, Taekjip Ha, Andreas Hartmann, Jelle Hendrix, Verena Hirschfeld, Christian G. Hübner, Thorsten Hugel, Dominik Kammerer, Hyun Seo Kang, Achillefs N. Kapanidis, Georg Krainer, Kevin Kramm, Edward A. Lemke, Eitan Lerner, Emmanuel Margeat, Kirsten Martens, Jens Michaelis, Jaba Mitra, Gabriel G. Moya Muñoz, Robert B. Quast, Nicole C. Robb, Michael Sattler, Michael Schlierf, Jonathan Schneider, Tim Schröder, Anna Sefer, Piau Siong Tan, Johann Thurn, Philip Tinnefeld, John van Noort, Shimon Weiss, Nicolas Wendler, Niels Zijlstra, Anders Barth, Claus A.M. Seidel, Don C. Lamb, Thorben Cordes (2023) Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins, Nature Methods 2023 20:4 20(4), p. 523-535, Nature Publishing Group, url, doi:10.1038/s41592-023-01807-0

Miao Yu, Maziar Heidari, Sofya Mikhaleva, Piau Siong Tan, Sara Mingu, Hao Ruan, Christopher D Reinkemeier, Agnieszka Obarska-Kosinska, Marc Siggel, Martin Beck, Gerhard Hummer, Edward A Lemke (2023) Visualizing the disordered nuclear transport machinery in situ, | Nature | 617, url, doi:10.1038/s41586-023-05990-0

2022

Christopher D Reinkemeier, Edward A Lemke (2022) Condensed, microtubule-coating thin organelles for orthogonal translation in mammalian cells., Journal of molecular biology, p. 167454, pubmed, doi:10.1016/j.jmb.2022.167454

Jasper J Michels, Mateusz Brzezinski, Tom Scheidt, Edward A Lemke, Sapun H Parekh (2022) Role of Solvent Compatibility in the Phase Behavior of Binary Solutions of Weakly Associating Multivalent Polymers., Biomacromolecules 23(1), p. 349-364, American Chemical Society (ACS), pubmed, doi:10.1021/acs.biomac.1c01301

2021

Bart W. Hoogenboom, Loren E. Hough, Edward A. Lemke, Roderick Y.H. Lim, Patrick R. Onck, Anton Zilman (2021) Physics of the nuclear pore complex: Theory, modeling and experiment, Physics Reports 921, p. 1-53, North-Holland, doi:10.1016/J.PHYSREP.2021.03.003

Tamas Lazar, Elizabeth Martínez-Pérez, Federica Quaglia, András Hatos, Lucía B. Chemes, Javier A. Iserte, Nicolás A. Méndez, Nicolás A. Garrone, Tadeo E. Saldaño, Julia Marchetti, Ana Julia Velez Rueda, Pau Bernadó, Martin Blackledge, Tiago N. Cordeiro, Eric Fagerberg, Julie D. Forman-Kay, Maria S. Fornasari, Toby J. Gibson, Gregory Neal W. Gomes, Claudiu C. Gradinaru, Teresa Head-Gordon, Malene Ringkjøbing Jensen, Edward A. Lemke, Sonia Longhi, Cristina Marino-Buslje, Giovanni Minervini, Tanja Mittag, Alexander Miguel Monzon, Rohit V. Pappu, Gustavo Parisi, Sylvie Ricard-Blum, Kiersten M. Ruff, Edoardo Salladini, Marie Skepö, Dmitri Svergun, Sylvain D. Vallet, Mihaly Varadi, Peter Tompa, Silvio C.E. Tosatto, Damiano Piovesan (2021) PED in 2021: A major update of the protein ensemble database for intrinsically disordered proteins, Nucleic Acids Research 49(D1), p. D404-D411, Oxford University Press, doi:10.1093/NAR/GKAA1021

Christopher D. Reinkemeier, Edward A. Lemke (2021) Dual film-like organelles enable spatial separation of orthogonal eukaryotic translation, Cell 184(19), p. 4886-4903.e21, Elsevier B.V., doi:10.1016/J.CELL.2021.08.001

Eitan Lerner, Anders Barth, Jelle Hendrix, Benjamin Ambrose, Victoria Birkedal, Scott C. Blanchard, Richard Börner, Hoi Sung Chung, Thorben Cordes, Timothy D. Craggs, Ashok A. Deniz, Jiajia Diao, Jingyi Fei, Ruben L. Gonzalez, Irina V. Gopich, Taekjip Ha, Christian A. Hanke, Gilad Haran, Nikos S. Hatzakis, Sungchul Hohng, Seok Cheol Hong, Thorsten Hugel, Antonino Ingargiola, Chirlmin Joo, Achillefs N. Kapanidis, Harold D. Kim, Ted Laurence, Nam Ki Lee, Tae Hee Lee, Edward A. Lemke, Emmanuel Margeat, Jens Michaelis, Xavier Michalet, Sua Myong, Daniel Nettels, Thomas Otavio Peulen, Evelyn Ploetz, Yair Razvag, Nicole C. Robb, Benjamin Schuler, Hamid Soleimaninejad, Chun Tang, Reza Vafabakhsh, Don C. Lamb, Claus A.M. Seidel, Shimon Weiss, Olga Boudker (2021) FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices, eLife 10, eLife Sciences Publications Ltd, doi:10.7554/ELIFE.60416

Christopher D. Reinkemeier, Christine Koehler, Paul F. Sauter, Nataliia V. Shymanska, Cecile Echalier, Anna Rutkowska, David W. Will, Carsten Schultz, Edward A. Lemke (2021) Synthesis and Evaluation of Novel Ring-Strained Noncanonical Amino Acids for Residue-Specific Bioorthogonal Reactions in Living Cells, Chemistry - A European Journal 27(19), p. 6094-6099, John Wiley and Sons Inc, doi:10.1002/CHEM.202100322

Giulia Paci, Joana Caria, Edward A. Lemke (2021) Cargo transport through the nuclear pore complex at a glance, Journal of Cell Science 134(2), Company of Biologists Ltd, doi:10.1242/JCS.247874

Jason D. Kahn, Edward A. Lemke, Rohit V. Pappu (2021) Faces, facets, and functions of biomolecular condensates driven by multivalent proteins and nucleic acids, Biophysical Journal 120(7), p. E1-E4, Biophysical Society, doi:10.1016/J.BPJ.2021.03.003

Miao Yu, Edward A. Lemke (2021) There is plenty of room in protein-RNA condensates, Biophysical Journal 120(7), p. 1121-1122, Biophysical Society, doi:10.1016/J.BPJ.2021.02.024

Lena Voith von Voithenberg, Anders Barth, Vanessa Trauschke, Benjamin Demarco, Swati Tyagi, Christine Koehler, Edward A. Lemke, Don C. Lamb (2021) Comparative analysis of the coordinated motion of Hsp70s from different organelles observed by single-molecule three-color FRET, Proceedings of the National Academy of Sciences of the United States of America 118(33), National Academy of Sciences, doi:10.1073/PNAS.2025578118

Philipp Werther, Klaus Yserentant, Felix Braun, Kristin Grußmayer, Vytautas Navikas, Miao Yu, Zhibin Zhang, Michael J. Ziegler, Christoph Mayer, Antoni J. Gralak, Marvin Busch, Weijie Chi, Frank Rominger, Aleksandra Radenovic, Xiaogang Liu, Edward A. Lemke, Tiago Buckup, Dirk Peter Herten, Richard Wombacher (2021) Bio-orthogonal Red and Far-Red Fluorogenic Probes for Wash-Free Live-Cell and Super-resolution Microscopy, ACS Central Science 7(9), p. 1561-1571, American Chemical Society, doi:10.1021/ACSCENTSCI.1C00703

Sara Mingu, Edward A. Lemke (2021) When two become one: Integrating FRET and EPR into one structural model, Biophysical Journal 120(21), p. 4637-4638, Biophysical Society, doi:10.1016/J.BPJ.2021.09.022

Christopher D. Reinkemeier, Edward A. Lemke (2021) Synthetic biomolecular condensates to engineer eukaryotic cells, Current Opinion in Chemical Biology 64, p. 174-181, Elsevier Ltd, doi:10.1016/j.cbpa.2021.08.005

2020

Giulia Paci, Tiantian Zheng, Joana Caria, Anton Zilman, Edward A. Lemke (2020) Molecular determinants of large cargo transport into the nucleus, eLife 9, p. 1-24, eLife Sciences Publications Ltd, doi:10.7554/ELIFE.55963

Christine Koehler, Gemma Estrada Girona, Christopher D. Reinkemeier, Edward A. Lemke (2020) Inducible Genetic Code Expansion in Eukaryotes, ChemBioChem 21(22), p. 3216-3219, Wiley-VCH Verlag, doi:10.1002/CBIC.202000338

Christopher D. Reinkemeier, Edward A. Lemke (2020) Raising the ribosomal repertoire, Nature Chemistry 12(6), p. 503-504, Nature Research, doi:10.1038/S41557-020-0476-6

Giorgia Celetti, Giulia Paci, Joana Caria, Virginia VanDelinder, George Bachand, Edward A. Lemke (2020) The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes, Journal of Cell Biology 219(1), Rockefeller University Press, doi:10.1083/JCB.201907157

Nike Heinß, Mikhail Sushkin, Miao Yu, Edward A. Lemke (2020) Multifunctionality of F-rich nucleoporins, Biochemical Society Transactions 48(6), p. 2603-2614, Portland Press Ltd, doi:10.1042/BST20200357

2019

Maolin Lu, Xiaochu Ma, Luis R. Castillo-Menendez, Jason Gorman, Nirmin Alsahafi, Utz Ermel, Daniel S. Terry, Michael Chambers, Dongjun Peng, Baoshan Zhang, Tongqing Zhou, Nick Reichard, Kevin Wang, Jonathan R. Grover, Brennan P. Carman, Matthew R. Gardner, Ivana Nikić-Spiegel, Akihiro Sugawara, James Arthos, Edward A. Lemke, Amos B. Smith, Michael Farzan, Cameron Abrams, James B. Munro, Adrian B. McDermott, Andrés Finzi, Peter D. Kwong, Scott C. Blanchard, Joseph G. Sodroski, Walther Mothes (2019) Associating HIV-1 envelope glycoprotein structures with states on the virus observed by smFRET, Nature 568(7752), p. 415-419, Nature Publishing Group, doi:10.1038/S41586-019-1101-Y

Aritra Chowdhury, Sergey A. Kovalenko, Iker Valle Aramburu, Piau Siong Tan, Nikolaus P. Ernsting, Edward A. Lemke (2019) Mechanism-Dependent Modulation of Ultrafast Interfacial Water Dynamics in Intrinsically Disordered Protein Complexes, Angewandte Chemie - International Edition 58(14), p. 4720-4724, Wiley-VCH Verlag, doi:10.1002/ANIE.201813354

Christopher D. Reinkemeier, Gemma Estrada Girona, Edward A. Lemke (2019) Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotes, Science 363(6434), American Association for the Advancement of Science, doi:10.1126/SCIENCE.AAW2644

Aritra Chowdhury, Miao Yu, Edward A. Lemke (2019) Phase Separation Comes of Age: From Phenomenology to Single Molecules, Molecular Cell 74(3), p. 413-415, Cell Press, doi:10.1016/J.MOLCEL.2019.04.016

2018

Christine Koehler, Edward A. Lemke (2018) MultiBacTAG-genetic code expansion using the baculovirus expression system in Sf21 cells, Methods in Molecular Biology 1728, p. 297-311, Humana Press Inc., doi:10.1007/978-1-4939-7574-7_19

Björn Hellenkamp, Sonja Schmid, Olga Doroshenko, Oleg Opanasyuk, Ralf Kühnemuth, Soheila Rezaei Adariani, Benjamin Ambrose, Mikayel Aznauryan, Anders Barth, Victoria Birkedal, Mark E. Bowen, Hongtao Chen, Thorben Cordes, Tobias Eilert, Carel Fijen, Christian Gebhardt, Markus Götz, Giorgos Gouridis, Enrico Gratton, Taekjip Ha, Pengyu Hao, Christian A. Hanke, Andreas Hartmann, Jelle Hendrix, Lasse L. Hildebrandt, Verena Hirschfeld, Johannes Hohlbein, Boyang Hua, Christian G. Hübner, Eleni Kallis, Achillefs N. Kapanidis, Jae Yeol Kim, Georg Krainer, Don C. Lamb, Nam Ki Lee, Edward A. Lemke, Brié Levesque, Marcia Levitus, James J. McCann, Nikolaus Naredi-Rainer, Daniel Nettels, Thuy Ngo, Ruoyi Qiu, Nicole C. Robb, Carlheinz Röcker, Hugo Sanabria, Michael Schlierf, Tim Schröder, Benjamin Schuler, Henning Seidel, Lisa Streit, Johann Thurn, Philip Tinnefeld, Swati Tyagi, Niels Vandenberk, Andrés Manuel Vera, Keith R. Weninger, Bettina Wünsch, Inna S. Yanez-Orozco, Jens Michaelis, Claus A.M. Seidel, Timothy D. Craggs, Thorsten Hugel (2018) Erratum to: Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study (Nature Methods, (2018), 15, 9, (669-676), 10.1038/s41592-018-0085-0), Nature Methods 15(11), p. 984, Nature Publishing Group, doi:10.1038/S41592-018-0193-X

Piau Siong Tan, Iker Valle Aramburu, Davide Mercadante, Swati Tyagi, Aritra Chowdhury, Daniel Spitz, Sarah L. Shammas, Frauke Gräter, Edward A. Lemke (2018) Two Differential Binding Mechanisms of FG-Nucleoporins and Nuclear Transport Receptors, Cell Reports 22(13), p. 3660-3671, Elsevier B.V., doi:10.1016/J.CELREP.2018.03.022

Robin L. Brabham, Richard J. Spears, Julia Walton, Swati Tyagi, Edward A. Lemke, Martin A. Fascione (2018) Palladium-unleashed proteins: Gentle aldehyde decaging for site-selective protein modification, Chemical Communications 54(12), p. 1501-1504, Royal Society of Chemistry, doi:10.1039/C7CC07740H

Eszter Kozma, Giulia Paci, Gemma Estrada Girona, Edward A. Lemke, Péter Kele (2018) Fluorogenic tetrazine-siliconrhodamine probe for the labeling of noncanonical amino acid tagged proteins, Methods in Molecular Biology 1728, p. 337-363, Humana Press Inc., doi:10.1007/978-1-4939-7574-7_22

Attila Kormos, Christine Koehler, Eszter A. Fodor, Zsófia R. Rutkai, Madison E. Martin, Gábor Mező, Edward A. Lemke, Péter Kele (2018) Bistetrazine-Cyanines as Double-Clicking Fluorogenic Two-Point Binder or Crosslinker Probes, Chemistry - A European Journal 24(35), p. 8841-8847, Wiley-VCH Verlag, doi:10.1002/CHEM.201800910

Björn Hellenkamp, Sonja Schmid, Olga Doroshenko, Oleg Opanasyuk, Ralf Kühnemuth, Soheila Rezaei Adariani, Benjamin Ambrose, Mikayel Aznauryan, Anders Barth, Victoria Birkedal, Mark E. Bowen, Hongtao Chen, Thorben Cordes, Tobias Eilert, Carel Fijen, Christian Gebhardt, Markus Götz, Giorgos Gouridis, Enrico Gratton, Taekjip Ha, Pengyu Hao, Christian A. Hanke, Andreas Hartmann, Jelle Hendrix, Lasse L. Hildebrandt, Verena Hirschfeld, Johannes Hohlbein, Boyang Hua, Christian G. Hübner, Eleni Kallis, Achillefs N. Kapanidis, Jae Yeol Kim, Georg Krainer, Don C. Lamb, Nam Ki Lee, Edward A. Lemke, Brié Levesque, Marcia Levitus, James J. McCann, Nikolaus Naredi-Rainer, Daniel Nettels, Thuy Ngo, Ruoyi Qiu, Nicole C. Robb, Carlheinz Röcker, Hugo Sanabria, Michael Schlierf, Tim Schröder, Benjamin Schuler, Henning Seidel, Lisa Streit, Johann Thurn, Philip Tinnefeld, Swati Tyagi, Niels Vandenberk, Andrés Manuel Vera, Keith R. Weninger, Bettina Wünsch, Inna S. Yanez-Orozco, Jens Michaelis, Claus A.M. Seidel, Timothy D. Craggs, Thorsten Hugel (2018) Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study, Nature Methods 15(9), p. 669-676, Nature Publishing Group, doi:10.1038/S41592-018-0085-0

Dibyendu Kumar Das, Ramesh Govindan, Ivana Nikić-Spiegel, Florian Krammer, Edward A. Lemke, James B. Munro (2018) Direct Visualization of the Conformational Dynamics of Single Influenza Hemagglutinin Trimers, Cell 174(4), p. 926-937.e12, Cell Press, doi:10.1016/J.CELL.2018.05.050

Sofya Mikhaleva, Edward A. Lemke (2018) Beyond the Transport Function of Import Receptors: What's All the FUS about?, Cell 173(3), p. 549-553, Cell Press, doi:10.1016/J.CELL.2018.04.002

Gustavo Fuertes, Niccolo Banterle, Kiersten M. Ruff, Aritra Chowdhury, Rohit V. Pappu, Dmitri I. Svergun, Edward A. Lemke (2018) Comment on “Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water”, Science 361(6405), American Association for the Advancement of Science, doi:10.1126/SCIENCE.AAU8230

Piau Siong Tan, Edward A. Lemke (2018) Probing Differential Binding Mechanisms of Phenylalanine-Glycine-Rich Nucleoporins by Single-Molecule FRET, Methods in Enzymology 611, p. 327-346, Academic Press Inc., doi:10.1016/BS.MIE.2018.08.034

2017

E. Kozma, G. Estrada Girona, G. Paci, E. A. Lemke, P. Kele (2017) Bioorthogonal double-fluorogenic siliconrhodamine probes for intracellular super-resolution microscopy, Chemical Communications 53(50), p. 6696-6699, Royal Society of Chemistry, url, doi:10.1039/C7CC02212C

Kalle Kipper, Ebba G. Lundius, Vladimir Ćurić, Ivana Nikić, Manfred Wiessler, Edward A. Lemke, Johan Elf (2017) Application of Noncanonical Amino Acids for Protein Labeling in a Genomically Recoded Escherichia coli, ACS Synthetic Biology 6(2), p. 233-255, American Chemical Society, doi:10.1021/ACSSYNBIO.6B00138

Kapil Gupta, Aleksandra A. Watson, Tiago Baptista, Elisabeth Scheer, Anna L. Chambers, Christine Koehler, Juan Zou, Ima Obong-Ebong, Eaazhisai Kandiah, Arturo Temblador, Adam Round, Eric Forest, Petr Man, Christoph Bieniossek, Ernest D. Laue, Edward A. Lemke, Juri Rappsilber, Carol V. Robinson, Didier Devys, Làszlò Tora, Imre Berger (2017) Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID, eLife 6, eLife Sciences Publications Ltd, doi:10.7554/ELIFE.30395

Gustavo Fuertes, Niccolò Banterle, Kiersten M. Ruff, Aritra Chowdhury, Davide Mercadante, Christine Koehler, Michael Kachala, Gemma Estrada Girona, Sigrid Milles, Ankur Mishra, Patrick R. Onck, Frauke Gräter, Santiago Esteban-Martín, Rohit V. Pappu, Dmitri I. Svergun, Edward A. Lemke (2017) Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements, Proceedings of the National Academy of Sciences of the United States of America 114(31), p. E6342-E6351, National Academy of Sciences, doi:10.1073/PNAS.1704692114

Giulia Paci, Edward A. Lemke (2017) Shining a Light on Phase Separation in the Cell, Cell 168(1-2), p. 11-13, Cell Press, doi:10.1016/J.CELL.2016.12.018

Svetlana Kucher, Sergei Korneev, Swati Tyagi, Ronja Apfelbaum, Dina Grohmann, Edward A. Lemke, Johann P. Klare, Heinz Jürgen Steinhoff, Daniel Klose (2017) Orthogonal spin labeling using click chemistry for in vitro and in vivo applications, Journal of Magnetic Resonance 275, p. 38-45, Academic Press Inc., doi:10.1016/J.JMR.2016.12.001

Volkan Sakin, Janina Hanne, Jessica Dunder, Maria Anders-Össwein, Vibor Laketa, Ivana Nikić, Hans Georg Kräusslich, Edward A. Lemke, Barbara Müller (2017) A Versatile Tool for Live-Cell Imaging and Super-Resolution Nanoscopy Studies of HIV-1 Env Distribution and Mobility, Cell Chemical Biology 24(5), p. 635-645.e5, Elsevier Ltd, doi:10.1016/J.CHEMBIOL.2017.04.007

Orsolya Demeter, Attila Kormos, Christine Koehler, Gábor Mezö, Krisztina Németh, Eszter Kozma, Levente B. Takács, Edward A. Lemke, Péter Kele (2017) Bisazide Cyanine Dyes as Fluorogenic Probes for Bis-Cyclooctynylated Peptide Tags and as Fluorogenic Cross-Linkers of Cyclooctynylated Proteins, Bioconjugate Chemistry 28(5), p. 1552-1559, American Chemical Society, doi:10.1021/ACS.BIOCONJCHEM.7B00178

Davide Mercadante, Johannes A. Wagner, Iker V. Aramburu, Edward A. Lemke, Frauke Gräter (2017) Sampling Long- versus Short-Range Interactions Defines the Ability of Force Fields to Reproduce the Dynamics of Intrinsically Disordered Proteins, Journal of Chemical Theory and Computation 13(9), p. 3964-3974, American Chemical Society, doi:10.1021/ACS.JCTC.7B00143

John B. Warner, Kiersten M. Ruff, Piau Siong Tan, Edward A. Lemke, Rohit V. Pappu, Hilal A. Lashuel (2017) Monomeric Huntingtin Exon 1 Has Similar Overall Structural Features for Wild-Type and Pathological Polyglutamine Lengths, Journal of the American Chemical Society 139(41), p. 14456-14469, American Chemical Society, doi:10.1021/JACS.7B06659

Iker Valle Aramburu, Edward A. Lemke (2017) Floppy but not sloppy: Interaction mechanism of FG-nucleoporins and nuclear transport receptors, Seminars in Cell and Developmental Biology 68, p. 34-41, Elsevier Ltd, doi:10.1016/J.SEMCDB.2017.06.026

2016

Volkan Sakin, Giulia Paci, Edward A. Lemke, Barbara Müller (2016) Labeling of virus components for advanced, quantitative imaging analyses, FEBS Letters, p. 1896-1914, Wiley Blackwell, doi:10.1002/1873-3468.12131

Niccolò Banterle, Edward A. Lemke (2016) Nanoscale devices for linkerless long-term single-molecule observation, Current Opinion in Biotechnology 39, p. 105-112, Elsevier Ltd, doi:10.1016/J.COPBIO.2016.02.013

Edward A. Lemke (2016) The Multiple Faces of Disordered Nucleoporins, Journal of Molecular Biology 428(10), p. 2011-2024, Academic Press, doi:10.1016/J.JMB.2016.01.002

Eszter Kozma, Ivana Nikić, Balázs R. Varga, Iker Valle Aramburu, Jun Hee Kang, Oliver T. Fackler, Edward A. Lemke, Péter Kele (2016) Hydrophilic trans-Cyclooctenylated Noncanonical Amino Acids for Fast Intracellular Protein Labeling, ChemBioChem, p. 1518-1524, Wiley-VCH Verlag, doi:10.1002/CBIC.201600284

Christine Koehler, Paul F. Sauter, Mirella Wawryszyn, Gemma Estrada Girona, Kapil Gupta, Jonathan J.M. Landry, Markus Hsi Yang Fritz, Ksenija Radic, Jan Erik Hoffmann, Zhuo A. Chen, Juan Zou, Piau Siong Tan, Bence Galik, Sini Junttila, Peggy Stolt-Bergner, Giancarlo Pruneri, Attila Gyenesei, Carsten Schultz, Moritz Bosse Biskup, Hueseyin Besir, Vladimir Benes, Juri Rappsilber, Martin Jechlinger, Jan O. Korbel, Imre Berger, Stefan Braese, Edward A. Lemke (2016) Genetic code expansion for multiprotein complex engineering, Nature Methods 13(12), p. 997-1000, Nature Publishing Group, doi:10.1038/NMETH.4032

Gergely Knorr, Eszter Kozma, András Herner, Edward A. Lemke, Péter Kele (2016) New Red-Emitting Tetrazine-Phenoxazine Fluorogenic Labels for Live-Cell Intracellular Bioorthogonal Labeling Schemes, Chemistry - A European Journal 22(26), p. 8972-8979, Wiley-VCH Verlag, doi:10.1002/CHEM.201600590

Ivana Nikić, Gemma Estrada Girona, Jun Hee Kang, Giulia Paci, Sofya Mikhaleva, Christine Koehler, Nataliia V. Shymanska, Camilla Ventura Santos, Daniel Spitz, Edward A. Lemke (2016) Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy, Angewandte Chemie - International Edition 55(52), p. 16172-16176, Wiley-VCH Verlag, doi:10.1002/ANIE.201608284

2015

Swati Tyagi, Edward A. Lemke (2015) Single-molecule FRET and crosslinking studies in structural biology enabled by noncanonical amino acids, Current Opinion in Structural Biology 32, p. 66-73, Elsevier Ltd, doi:10.1016/J.SBI.2015.02.009

Ivana Nikic, Jun Hee Kang, Gemma Estrada Girona, Iker Valle Aramburu, Edward A. Lemke (2015) Labeling proteins on live mammalian cells using click chemistry, Nature Protocols 10(5), p. 780-791, Nature Publishing Group, doi:10.1038/NPROT.2015.045

Alexander Von Appen, Jan Kosinski, Lenore Sparks, Alessandro Ori, Amanda L. DiGuilio, Benjamin Vollmer, Marie Therese Mackmull, Niccolo Banterle, Luca Parca, Panagiotis Kastritis, Katarzyna Buczak, Shyamal Mosalaganti, Wim Hagen, Amparo Andres-Pons, Edward A. Lemke, Peer Bork, Wolfram Antonin, Joseph S. Glavy, Khanh Huy Bui, Martin Beck (2015) In situ structural analysis of the human nuclear pore complex, Nature 526(7571), p. 140-143, Nature Publishing Group, doi:10.1038/NATURE15381

Ivana Nikić, Edward A. Lemke (2015) Genetic code expansion enabled site-specific dual-color protein labeling: Superresolution microscopy and beyond, Current Opinion in Chemical Biology 28, p. 164-173, Elsevier Ltd, doi:10.1016/J.CBPA.2015.07.021

Jan Erik Hoffmann, Tilman Plass, Ivana Nikic, Iker Valle Aramburu, Christine Koehler, Hartmut Gillandt, Edward A. Lemke, Carsten Schultz (2015) Highly Stable trans-Cyclooctene Amino Acids for Live-Cell Labeling, Chemistry - A European Journal 21(35), p. 12266-12270, Wiley-VCH Verlag, doi:10.1002/CHEM.201501647

Ingrid C. Vreja, Ivana Nikić, Fabian Göttfert, Mark Bates, Katharina Kröhnert, Tiago F. Outeiro, Stefan W. Hell, Edward A. Lemke, Silvio O. Rizzoli (2015) Super-resolution Microscopy of Clickable Amino Acids Reveals the Effects of Fluorescent Protein Tagging on Protein Assemblies, ACS Nano 9(11), p. 11034-11041, American Chemical Society, doi:10.1021/ACSNANO.5B04434

Davide Mercadante, Sigrid Milles, Gustavo Fuertes, Dmitri I. Svergun, Edward A. Lemke, Frauke Gräter (2015) Kirkwood-Buff Approach Rescues Overcollapse of a Disordered Protein in Canonical Protein Force Fields, Journal of Physical Chemistry B 119(25), p. 7975-7984, American Chemical Society, doi:10.1021/ACS.JPCB.5B03440

Sigrid Milles, Davide Mercadante, Iker Valle Aramburu, Malene Ringkjøbing Jensen, Niccolò Banterle, Christine Koehler, Swati Tyagi, Jane Clarke, Sarah L. Shammas, Martin Blackledge, Frauke Gräter, Edward A. Lemke (2015) Plasticity of an Ultrafast Interaction between Nucleoporins and Nuclear Transport Receptors, Cell 163(3), p. 734-745, Cell Press, doi:10.1016/J.CELL.2015.09.047

Elise Delaforge, Sigrid Milles, Guillaume Bouvignies, Denis Bouvier, Stephane Boivin, Nicola Salvi, Damien Maurin, Anne Martel, Adam Round, Edward A. Lemke, Malene Ringkjøbing Jensen, Darren J. Hart, Martin Blackledge (2015) Large-Scale Conformational Dynamics Control H5N1 Influenza Polymerase PB2 Binding to Importin α, Journal of the American Chemical Society 137(48), p. 15122-15134, American Chemical Society, doi:10.1021/JACS.5B07765

Johannes A. Wagner, Davide Mercadante, Ivana Nikic, Edward A. Lemke, Frauke Gräter (2015) Origin of Orthogonality of Strain-Promoted Click Reactions, Chemistry - A European Journal 21(35), p. 12431-12435, Wiley-VCH Verlag, doi:10.1002/CHEM.201501727

2014

Ivana Nikic̈, Tilman Plass, Oliver Schraidt, Jȩdrzej Szymański, John A.G. Briggs, Carsten Schultz, Edward A. Lemke (2014) Minimal tags for rapid dual-color live-cell labeling and super-resolution microscopy, Angewandte Chemie - International Edition 53(8), p. 2245-2249, doi:10.1002/ANIE.201309847

Edward A. Lemke (2014) The exploding genetic code, ChemBioChem 15(12), p. 1691-1694, Wiley-VCH Verlag, doi:10.1002/CBIC.201402362

Swati Tyagi, Virginia Vandelinder, Niccolò Banterle, Gustavo Fuertes, Sigrid Milles, Morgane Agez, Edward A. Lemke (2014) Continuous throughput and long-term observation of single-molecule FRET without immobilization, Nature Methods 11(3), p. 297-300, Nature Publishing Group, doi:10.1038/NMETH.2809

Karolina Pavic, Pablo Rios, Kristina Dzeyk, Christine Koehler, Edward A. Lemke, Maja Köhn (2014) Unnatural amino acid mutagenesis reveals dimerization as a negative regulatory mechanism of VHR's phosphatase activity, ACS Chemical Biology 9(7), p. 1451-1459, American Chemical Society, doi:10.1021/CB500240N

Matthew Dunne, Haydyn D.T. Mertens, Vasiliki Garefalaki, Cy M. Jeffries, Andrew Thompson, Edward A. Lemke, Dmitri I. Svergun, Melinda J. Mayer, Arjan Narbad, Rob Meijers (2014) The CD27L and CTP1L Endolysins Targeting Clostridia Contain a Built-in Trigger and Release Factor, PLoS Pathogens 10(7), Public Library of Science, doi:10.1371/JOURNAL.PPAT.1004228

András Herner, Gemma Estrada Girona, Ivana Nikić, Mihály Kállay, Edward A. Lemke, Péter Kele (2014) New generation of bioorthogonally applicable fluorogenic dyes with visible excitations and large stokes shifts, Bioconjugate Chemistry 25(7), p. 1370-1374, American Chemical Society, doi:10.1021/BC500235P

Sigrid Milles, Edward A. Lemke (2014) Mapping multivalency and differential affinities within large intrinsically disordered protein complexes with segmental motion analysis, Angewandte Chemie - International Edition 53(28), p. 7364-7367, Wiley-VCH Verlag, doi:10.1002/ANIE.201403694

2013

Swati Tyagi, Edward A. Lemke (2013) Genetically Encoded Click Chemistry for Single-Molecule FRET of Proteins, Methods in Cell Biology 113, p. 169-187, Academic Press Inc., doi:10.1016/B978-0-12-407239-8.00009-4

Alessandro Ori, Niccolò Banterle, Murat Iskar, Amparo Andrés-Pons, Claudia Escher, Huy Khanh Bui, Lenore Sparks, Victor Solis-Mezarino, Oliver Rinner, Peer Bork, Edward A. Lemke, Martin Beck (2013) Cell type-specific nuclear pores: A case in point for context-dependent stoichiometry of molecular machines, Molecular Systems Biology 9(1), Blackwell Publishing Ltd, doi:10.1038/MSB.2013.4

Sigrid Milles, Edward A. Lemke (2013) What precision-protein-tuning and nano-resolved single molecule sciences can do for each other, BioEssays 35(1), p. 65-74, doi:10.1002/BIES.201200094

Sigrid Milles, Khanh Huy Bui, Christine Koehler, Mikhail Eltsov, Martin Beck, Edward A. Lemke (2013) Facilitated aggregation of FG nucleoporins under molecular crowding conditions, EMBO Reports 14(2), p. 178-183, doi:10.1038/EMBOR.2012.204

Gražvydas Lukinavičius, Keitaro Umezawa, Nicolas Olivier, Alf Honigmann, Guoying Yang, Tilman Plass, Veronika Mueller, Luc Reymond, Ivan R. Corrêa, Zhen Ge Luo, Carsten Schultz, Edward A. Lemke, Paul Heppenstall, Christian Eggeling, Suliana Manley, Kai Johnsson (2013) A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins, Nature Chemistry 5(2), p. 132-139, doi:10.1038/NCHEM.1546

András Herner, Ivana Nikić, Mihály Kállay, Edward A. Lemke, Péter Kele (2013) A new family of bioorthogonally applicable fluorogenic labels, Organic and Biomolecular Chemistry 11(20), p. 3297-3306, Royal Society of Chemistry, doi:10.1039/C3OB40296G

Niccolò Banterle, Khanh Huy Bui, Edward A. Lemke, Martin Beck (2013) Fourier ring correlation as a resolution criterion for super-resolution microscopy, Journal of Structural Biology 183(3), p. 363-367, doi:10.1016/J.JSB.2013.05.004

2012

Sigrid Milles, Swati Tyagi, Niccolò Banterle, Christine Koehler, Virginia Vandelinder, Tilman Plass, Adrian P. Neal, Edward A. Lemke (2012) Click strategies for single-molecule protein fluorescence, Journal of the American Chemical Society 134(11), p. 5187-5195, doi:10.1021/JA210587Q

Rajaraman Krishnan, Jessica L. Goodman, Samrat Mukhopadhyay, Chris D. Pacheco, Edward A. Lemke, Ashok A. Deniz, Susan Lindquist (2012) Conserved features of intermediates in amyloid assembly determine their benign or toxic states, Proceedings of the National Academy of Sciences of the United States of America 109(28), p. 11172-11177, doi:10.1073/PNAS.1209527109

Annika Borrmann, Sigrid Milles, Tilman Plass, Jan Dommerholt, Jorge M.M. Verkade, Manfred Wießler, Carsten Schultz, Jan C.M. van Hest, Floris L. van Delft, Edward A. Lemke (2012) Genetic Encoding of a Bicyclo[6.1.0]nonyne-Charged Amino Acid Enables Fast Cellular Protein Imaging by Metal-Free Ligation, ChemBioChem 13(14), p. 2094-2099, doi:10.1002/CBIC.201200407

Sigrid Milles, Christine Koehler, Yann Gambin, Ashok A. Deniz, Edward A. Lemke (2012) Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range, Molecular BioSystems 8(10), p. 2531-2534, Royal Society of Chemistry, doi:10.1039/C2MB25135C

Tilman Plass, Sigrid Milles, Christine Koehler, Jȩdrzej Szymański, Rainer Mueller, Manfred Wießler, Carsten Schultz, Edward A. Lemke (2012) Amino acids for diels-alder reactions in living cells, Angewandte Chemie - International Edition 51(17), p. 4166-4170, doi:10.1002/ANIE.201108231

2011

Edward A. Lemke, Carsten Schultz (2011) Principles for designing fluorescent sensors and reporters, Nature Chemical Biology 7(8), p. 480-483, Nature Publishing Group, doi:10.1038/NCHEMBIO.620

Tilman Plass, Sigrid Milles, Christine Koehler, Carsten Schultz, Edward A. Lemke (2011) Genetically encoded copper-free click chemistry, Angewandte Chemie - International Edition 50(17), p. 3878-3881, doi:10.1002/ANIE.201008178

Sigrid Milles, Edward A. Lemke (2011) Single molecule study of the intrinsically disordered FG-repeat nucleoporin 153, Biophysical Journal 101(7), p. 1710-1719, doi:10.1016/J.BPJ.2011.08.025

Yann Gambin, Virginia Vandelinder, Allan Chris M. Ferreon, Edward A. Lemke, Alex Groisman, Ashok A. Deniz (2011) Visualizing a one-way protein encounter complex by ultrafast single-molecule mixing, Nature Methods 8(3), p. 239-241, doi:10.1038/NMETH.1568

Edward A. Lemke (2011) Site-specific labeling of proteins for single-molecule fret measurements using genetically encoded ketone functionalities, Methods in Molecular Biology 751, p. 3-15, Humana Press Inc., doi:10.1007/978-1-61779-151-2_1

2010

Edward A. Lemke (2010) Precision control of cellular pathways with light, ChemBioChem 11(13), p. 1825-1827, doi:10.1002/CBIC.201000364

2009

Yann Gambin, Alexander Schug, Edward A. Lemke, Jason J. Lavinder, Allan Chris M. Ferreon, Thomas J. Magliery, José N. Onuchic, Ashok A. Deniz (2009) Direct single-molecule observation of a protein living in two opposed native structures, Proceedings of the National Academy of Sciences of the United States of America 106(25), p. 10153-10158, doi:10.1073/PNAS.0904461106

Edward A. Lemke, Yann Gambin, Virginia Vandelinder, Eric M. Brustad, Hsiao Wei Liu, Peter G. Schultz, Alex Groisman, Ashok A. Deniz (2009) Microfluidic device for single-molecule experiments with enhanced photostability, Journal of the American Chemical Society 131(38), p. 13610-13612, doi:10.1021/JA9027023

Soo Lee Hyun, Jiantao Guo, Edward A. Lemke, Romerson D. Dimla, Peter G. Schultz (2009) Genetic incorporation of a small, environmentally sensitive, fluorescent probe into proteins in Saccharomyces cerevisiae, Journal of the American Chemical Society 131(36), p. 12921-12923, doi:10.1021/JA904896S

Allan Chris M. Ferreon, Yann Gambin, Edward A. Lemke, Ashok A. Deniz (2009) Interplay of α-synuclein binding and conformational switching probed by single-molecule fluorescence, Proceedings of the National Academy of Sciences of the United States of America 106(14), p. 5645-5650, doi:10.1073/PNAS.0809232106

2008

Ashok A. Deniz, Samrat Mukhopadhyay, Edward A. Lemke (2008) Single-molecule biophysics: At the interface of biology, physics and chemistry, Journal of the Royal Society Interface 5(18), p. 15-45, Royal Society, doi:10.1098/RSIF.2007.1021

Eric M. Brustad, Edward A. Lemke, Peter G. Schultz, Ashok A. Deniz (2008) A general and efficient method for the site-specific dual-labeling of proteins for single molecule fluorescence resonance energy transfer, Journal of the American Chemical Society 130(52), p. 17664-17665, doi:10.1021/JA807430H

2007

A. W. Henkel, P. S. Dittrich, T. W. Groemer, E. A. Lemke, J. Klingauf, H. W. Klafki, P. Lewczuk, H. Esselmann, P. Schwille, J. Kornhuber, J. Wiltfang (2007) Immune complexes of auto-antibodies against A beta 1-42 peptides patrol cerebrospinal fluid of non-Alzheimer's patients, Molecular psychiatry 12(6), p. 601-610, Mol Psychiatry, url, doi:10.1038/SJ.MP.4001947

Samrat Mukhopadhyay, Rajaraman Krishnan, Edward A. Lemke, Susan Lindquist, Ashok A. Deniz (2007) A natively unfolded yeast prion monomer adopts an ensemble of collapsed and rapidly fluctuating structures, Proceedings of the National Academy of Sciences of the United States of America 104(8), p. 2649-2654, doi:10.1073/PNAS.0611503104

Edward A. Lemke, Daniel Summerer, Bernhard H. Geierstanger, Scott M. Brittain, Peter G. Schultz (2007) Control of protein phosphorylation with a genetically encoded photocaged amino acid, Nature Chemical Biology 3(12), p. 769-772, Nature Publishing Group, doi:10.1038/NCHEMBIO.2007.44

2006

Shigeo Takamori, Matthew Holt, Katinka Stenius, Edward A. Lemke, Mads Grønborg, Dietmar Riedel, Henning Urlaub, Stephan Schenck, Britta Brügger, Philippe Ringler, Shirley A. Müller, Burkhard Rammner, Frauke Gräter, Jochen S. Hub, Bert L. De Groot, Gottfried Mieskes, Yoshinori Moriyama, Jürgen Klingauf, Helmut Grubmüller, John Heuser, Felix Wieland, Reinhard Jahn (2006) Molecular Anatomy of a Trafficking Organelle, Cell 127(4), p. 831-846, Elsevier B.V., doi:10.1016/J.CELL.2006.10.030

2005

Edward A. Lemke, Jurgen Klingauf (2005) Single synaptic vesicle tracking in individual hippocampal boutons at rest and during synaptic activity, Journal of Neuroscience 25(47), p. 11034-11044, doi:10.1523/JNEUROSCI.2971-05.2005

Randolf Jordan, Edward A. Lemke, Jurgen Klingauf (2005) Visualization of synaptic vesicle movement in intact synaptic boutons using fluorescence fluctuation spectroscopy, Biophysical Journal 89(3), p. 2091-2102, Biophysical Society, doi:10.1529/BIOPHYSJ.105.061663

2003

Ranjan Chakraborty, Edward A. Lemke, Zenghua Cao, Phillip E. Klebba, Dick Van Der Helm (2003) Identification and mutational studies of conserved amino acids in the outer membrane receptor protein, FepA, which affect transport but not binding of ferric-enterobactin in Escherichia coli, BioMetals 16(4), p. 507-518, doi:10.1023/A:1023485632520